A fluorescent mushroom distributed in Brazil will emit green fluorescence in the dark, and Russian and Brazilian researchers are expected to use other biochemical experiments by analyzing the luminescence mechanism of this mushroom and synthesizing fluorescein molecules.
In some parts of Brazil under the palm trees there is a local people commonly known as “coconut flower” fluorescent mushrooms. In the sun this mushroom is yellow and white color, but in the night or dark cave it can emit green fluorescence, attracting insects to help spread the spore propagation. How is this mushroom glowing? The Institute of Bioorganic Chemistry of the Russian Academy of Sciences and the Brazilian colleagues have studied this.
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Researchers reported in the new issue of “Progress in Science” that they analyzed the cells of the mushroom, extracted the proteins and fluorescein molecules associated with the emission of fluorescence, and analyzed these substances by gas chromatography and mass spectrometry. Under the chemical changes that occur. It was eventually found that some of the protein molecules in the mushroom cells used the intracellular oxygen and adenosine triphosphate to oxidize their own specific fluorescein to emit green light.
The researchers also found that the above-mentioned fluorescein molecules consist of two important fragments. They mimicked the structure of these two fragments and synthesized six kinds of fluorescein molecular fragments with chemical fluorescent substrate-alfa pyrone and used them to replace the natural fluorescein molecular fragments of mushrooms one by one. It was found that there were five artificial fragments Natural fragments collaborate to produce orange, yellow, light green, blue, and light blue in the absence of mushrooms, individually cultured.
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Researchers say the study has made them not only aware of the mechanism by which fluorescent mushrooms emit light in the dark, but also have a way to control their fluorescent color. It is expected that the cells will emit light in animal and plant cell experiments in order to observe their biochemistry Variety. In addition, the team is also prepared to modify the fluorescent mushroom gene, so that the synthesis of all the luciferin molecular fragments, in order to cultivate the colorful “rainbow” mushrooms.
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